Counting Tissue Samples
BIOSOL/BIOSCINT
- Place up to 200 mg of tissue, or 1 ml of blood, in a glass scintillation vial. Ground or minced tissue will dissolve more rapidly. Avoid adhesion of the sample to the bottom of the vial, as this will extend the digestion time.
- Add 1 ml of Biosol. Agitate gently (do not vortex).
- Incubate in shaking water bath at 50°C for 1-4 hours, until clear.
- If necessary (for blood or other pigmented samples) decolorize with 0.2 ml of 30% H2O2. Cap loosely and incubate at 50°C 1 hour.
- Cool to room temperature, add 10 ml of Bioscint and count.
SOLUSOL
- Place up to 100 mg of tissue, or 0.5 ml of blood, in a glass scintillation vial. Ground or minced tissue will dissolve more rapidly. Avoid adhesion of the sample to the bottom of the vial, as this will extend the digestion time.
- Add 0.2-0.4 ml of Solusol. For blood samples, use 1-5 volumes Solusol per volume of sample. Agitate gently (do not vortex).
- Incubate at 50°C for 1-2 hours, or at room temperature for 3-5 hours, until clear. Blood samples may require longer digestions (up to 24 hours in some cases).
- If necessary (for blood or other pigmented samples) decolorize with 2 volumes of 20% benzoyl peroxide in toluene. Cap loosely and incubate at 50°C 30 minutes. Lightly colored samples may be bleached by UV or sunlight exposure.
- Cool to room temperature, add 10 ml of Soluscint XR and count.
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