Overview of the Paraffin Technique

Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope. The procedures designed to prepare the tissue for sectioning are collectively known as tissue processing. First, the sample is dehydrated by immersion in a series of aqueous alcohol solutions gradually moving to pure alcohol. The tissue is then soaked in an appropriate solvent to remove the alcohol. Finally the tissue is embedded in paraffin wax, which enables the cutting of sections of between 3 and 10 microns thickness. The movement through the series of baths in tissue processing occurs either by hand or by means of an automated processor. The table below gives a typical tissue schedule for an automated processor. The optimum protocol for any particular sample and apparatus may vary.

Automated Tissue Processing Schedule

Process Bath18 Hour Cycle (Time in Hours)24 Hour Cycle (Time in Hours)
70% ethanol1.52
80% ethanol1.52
95% ethanol1.52
Absolute alcohol1.52
Absolute alcohol1.52
Alcohol/Histo-Clear (v/V)1.52
HIsto-Clear1.52
Histo-Clear1.52
Histo-Clear1.52
Paraffin1.52
Paraffin1.52
Paraffin1.52

 

Complete Process for Tissue Preparation
An overview of the complete process of tissue preparation for viewing by light microscopy, from fixation to mounting.


NEXT TOPIC: Suggested Procedures for Processing Fixed Tissue

Shopping cart

0 Items $0.00