Uneven Staining

Uneven Staining

 

Uneven staining is almost always the result if insufficient agitation during staining.  The gel is initially more dense than the staining solution, and will tend to sink to the bottom of the dish.  Some portions of the gel will have stain under them, others will not, and the gel will show darker and lighter areas as a result.  

5X Protein Loading Buffer can help you attain clear, consistent staining.

 

To recover this gel, simply continue the staining process with agitation (on a shaker), and the staining will become uniform.

 

 

Protogel Quick Cast- ready to run in 20 minutes!

ProtoBlue Safe Colloidal Coomassie Stain

ProtoBlue Safe Colloidal Coomassie Stain

ProtoGel-  for the best resolution SDS-PAGE

 

 

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