Decalcifying Tissue for Histological Processing

The removal of calcium deposits is essential for good embedding procedure. Decalcification is usually carried out between the fixation and processing steps. Bone must obviously be processed in this way, but other tissues may also contain calcified areas. A variety of agents or techniques have been developed to decalcify tissues, each with advantages and disadvantages.…

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Overview of Western Blotting

Western blotting apparatus

Proteins can also be detected immunologically following electrophoresis, a technique known as Western blotting. This method relies on the fact that most epitopes (sites recognized by antibodies, generally comprising several amino acids) are still recognizable following denaturing of the protein with SDS and binding to the surface of a membrane. The Western Blotting apparatus. Proteins…

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