Fundamental Principles of Electrophoresis

The Agarose Matrix

A natural colloid extracted from seaweed, agarose is a linear polysaccharide made up of the repeating unit agarobiose, which consists of alternati

Electrophoresis Buffers--The Henderson-Hasselbalch Equation

In its simplest form, a buffered solution contains a mixture of a weak acid and its conjugate base.

Electrophoresis Buffers-Choosing the Right Buffer

Several factors to consider when choosing a buffer include:

Homogeneous Buffer Systems

In a homogeneous buffer system, the identity and concentration of buffer components are the same in the gel and the tanks.

Multiphasic Buffer Systems

Employing gel and buffer discontinuities to produce sharp separation among sample components, multiphasic electrophoresis design can improve the r

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