Fundamental Principles of Electrophoresis

The Electrophoresis Matrix

In gel electrophoresis the matrix forces sample components to separate by size, as they move through its porous structure.

The Polyacrylamide Matrix

Polyacrylamide gels are formed by the polymerization of acrylamide in aqueous solution in the presence of small amounts of a bifunctional crosslinker. The crosslinker is usually methylenebisacrylamide (bis, or MBA).

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