Fundamental Principles of Electrophoresis

Buffer Additives-Hydrogen Bonding Agents

In most forms of electrophoresis the solution perfusing the gel matrix typically contains one or more substances in addition to the buffer salts.

Buffer Additives-Reducing Agents

Disulfide bonds between or within sample protein molecules can lead to the formation of aggregates as well as play a role in the binding of the sub

The Polyacrylamide Matrix-Buffer Strength

The buffer system in electrophoresis controls the pH of the gel, preventing damage to sample molecules and, in certain cases, controlling the ioniz


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