Applications of Denaturing DNA and RNA Gels

Using PAGE to Determine Nucleic Acid Molecular Weight

Molecular weight determination is the most basic use of denaturing polyacrylamide gel electrophoresis.

Maxam & Gilbert Sequencing

There are four chemical cleavage reactions at the core of the Maxam and Gilbert sequencing system.

Sanger Sequencing

In Sanger dideoxy terminator sequencing, the sample DNA is used as a template for a DNA polymerase.

Automated Sequencers

Automated sequencing systems make use of fluorescent dye labeling, in combination with laser scanning and computerized data acquisition and proces

Differential Display

Differential display is a powerful technique for detecting and quantitating changes in gene expression patterns between differently treated cells.


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