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  • Articles : Denaturing Gel and Sample Preparation

Denaturing Gel and Sample Preparation

Sample Prep for Denaturing PAGE of DNA

DNA samples for denaturing gel electrophoresis must be denatured prior to loading, to avoid time dependent denaturation artifacts on the gel.

Preparing Denaturing DNA & RNA Gels

Sequencing gels are poured between two glass plates separated by spacers. The spacers are typically no more than 0.2mm in thickness.

Pouring Sequencing Gels

Denaturing PAGE gels for DNA sequencing generally employ 6-8 M

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