Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H302 - Acute Toxicity-Oral (Category 4)
H313 - Acute Toxicity-Dermal (Category 5)
H333 - Acute Toxicity-Inhalation (Category 5)
2.2 Label Elements
| GHS Label Elements | ||
 | WARNING H302 - Harmful if swallowed | |
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Transfer Buffer Concentrate
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| Methanol | 5 | 67-56-1 | 200-659-6 | H225, H301, H311, H331, H371 |
| Tris-Base | 3 | 77-86-1 | 201-064-4 | H315, H319, H335 |
| Glycine | 14.4 | 56-40-6 | 200-272-2 | N.A. |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
Call a physician immediately if significant amounts have been swallowed. Give large amounts of water or milk to drink for dilution effect. Do not induce vomiting.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Methanol:
Irritation of the respiratory tract and mucous membranes. For central nervous system symptoms which may occur due to exposure by inhalation, see Ingestion.
Tris-Base:
Coughing, shortness of breath.
Glycine:
Coughing, soreness in the respiratory tract, chest tightening, difficulty breathing.
Ingestion
Methanol:
Effects may include excitation, euphoria, headache, dizziness, drowsiness, blurred vision, fatigue, tremors, convulsions, loss of consciousness, coma, respiratory arrest and death.
Tris-Base:
Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse, blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.
Glycine:
Nausea.
Skin
Methanol:
Exposure may cause symptoms similar to those listed under Ingestion.
Tris-Base:
Redness, itching, and pain.
Glycine:
Mild reddening.
Eyes
Methanol:
Irritation, redness, pain, and inflammation.
Tris-Base:
Redness, itching, and pain.
Glycine:
Mild reddening.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Use media appropriate to the primary cause of fire.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Thermal decomposition products may include toxic oxides of nitrogen and carbon.
Hazardous Decomposition Products
Burning may produce carbon monoxide, carbon dioxide, nitrogen oxides.
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using absorbents. Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with copious amounts of water.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area.
Incompatibles
Methanol:
Acetyl bromide, calcium carbide, chlorine, chromic anhydride, cyanuric chloride, dichloromethane, diethyl zinc, lead perchlorate, magnesium, metals, strong oxidizers, perchloric acid, phosphorous trioxide, potassium, sodium hypochlorite, sulfuric acid and zinc.
Tris-Base:
No incompatibility data found.
Glycine:
No incompatibility data found.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: Methanol
ACGIH Threshold Limit Value (TLV):
200 ppm
OSHA Permissable Exposure Limit (PEL):
None established
Component: Tris-Base
ACGIH Threshold Limit Value (TLV):
none established
OSHA Permissable Exposure Limit (PEL):
none established
Component: Glycine
ACGIH Threshold Limit Value (TLV):
15 mg/m3
OSHA Permissable Exposure Limit (PEL):
None established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures low. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator.
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Skin Protection
Wear protective gloves and clean body covering clothing.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Clear, colorless solution | b. Odor | None |
| c. Odor Threshold | N.A. | d. pH | 8.0 |
| e. Melting/Freezing Point (oC) | 0 | f. Boiling point (oC) | 104.4 |
| g. Flash Point (oC) | >98 | h. Evaporation Rate | 1.0 |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | N.A. |
| k. Vapor Pressure | Water | l. Vapor Density (Air = 1) | N.A. |
| m. Relative Density | 1.05 | n. Water Solubility | Soluble |
| o. Partition Coefficient n-octanol/water | Mixture | p. Autoignition Temperature (oC) | N.A. |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | No data available. |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | N.A. |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Not reactive under normal conditions of use and storage.
10.2 Chemical Stability
Stable under ordinary conditions of use and storage.
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
10.4 Conditions to Avoid
Heat, incompatibles.
10.5 Incompatible Materials
Methanol:
Acetyl bromide, calcium carbide, chlorine, chromic anhydride, cyanuric chloride, dichloromethane, diethyl zinc, lead perchlorate, magnesium, metals, strong oxidizers, perchloric acid, phosphorous trioxide, potassium, sodium hypochlorite, sulfuric acid and zinc.
Tris-Base:
No incompatibility data found.
Glycine:
No incompatibility data found.
10.6 Hazardous Decomposition Products
Burning may produce carbon monoxide, carbon dioxide, nitrogen oxides.
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
> 5000
Dermal Rabbit LD50 (mg/kg)
> 5000
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| Methanol | No | No | None |
| Tris-Base | No | No | None |
| Glycine | No | No | None |
Potential Health Effects
Inhalation
Methanol
May irritate the respiratory tract and mucuous membranes. Exposure may cause central nervous system symptoms similar to those listed under Ingestion.
Tris-Base
Causes irritation to the respiratory tract.
Glycine
High concentrations of dust may be slightly irritating to the lungs.
Ingestion
Methanol
Primary toxic effects are metabolic acidosis and visual system damage. Visual system damage may progress from visual blurring to complete blindness. May cause harmful central nervous system effects which may be delayed.
Tris-Base
Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
Glycine
Large oral doses may cause nausea.
Skin
Methanol
Absorption from prolonged or massive skin contact may cause poisoning. Repeated or prolonged contact may result in defatting, redness, itching, inflammation, cracking and possible secondary infection. Exposure may cause symptoms similar to those listed under ingestion.
Tris-Base
Causes irritation to the skin.
Glycine
May cause mild irritation to the skin.
Eyes
Methanol
Exposure to liquid, vapors, fumes or mists may cause irritation. Direct contact may cause irritation, pain, corneal inflammation and possible corneal damage.
Tris-Base
Causes irritation to the eyes.
Glycine
May cause mild irritation to the eyes.
Carcinogenicity
Methanol
This component is not listed as a carcinogen by NTP or IARC.
Tris-Base
Not listed as a carcinogen by NTP or IARC.
Glycine
According to definitions of the U.S. Hazard Communication Standard and the Canadian WHMIS Regulation, this material is not listed on the NTP, IARC, ACGIH, or OSHA carcinogen lists, and there are no studies implicating components as cancer causing agents in humans or animals.
Mutagenicity
Methanol
No information available.
Tris-Base
No information found.
Glycine
No information found.
Reproductive Toxicity
Methanol
Possible reproductive hazard.
Tris-Base
No information found.
Glycine
No information found.
Teratogenic Effects
Methanol
No information available.
Tris-Base
No information found.
Glycine
No information found.
Routes of Entry
Methanol
Inhalation, ingestion, or skin contact.
Tris-Base
Ingestion.
Glycine
Inhalation of dust.
Target Organ Statement
Methanol
Chronic intoxication may cause degenerative changes in liver, kidneys, brain, gastrointestinal tract, and heart muscle. Persons with pre-existing liver impairment, skin and respiratory disorders may be at an increased risk from exposure.
Tris-Base
No information available.
Glycine
Persons with pre-existing skin or lung disorders may be more susceptible to irritation effects.
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: Methanol
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (96hr, bluegill) 15400 mg/L | EC50 (48 hr, daphnia) > 10000 mg/L | EC50 (96 hr) 22000 mg/L | IC50 (3hr) > 1000 mg/L |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | IC50 (3 day, sativa) 41000 mg/L | No data | No data | No data |
COMPONENT: Tris-Base
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 460mg/l (Golden ide) | EC50: 59.8 mg/L (Daphnia) | EC50: 473mg/l @ 48 hrs | CE50>1000mg/L (3hrs) |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
COMPONENT: Glycine
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
12.2 Persistence and Degradability
Methanol
Readily biodegradable (95% elimination in 20 days)
Tris-Base
Readily Biodegradable (>97% degradation at 28 days)
Glycine
No data
12.3 Bioaccumulative Potential
Methanol
BCF<10
Tris-Base
No data
Glycine
No data
12.4 Mobility in Soil
Methanol
Koc <1
Tris-Base
Log Koc 1.57-1.85
Glycine
No data
12.5 Results of PBT and vPvB Assessment
Methanol
not PBT or vPvB
Tris-Base
Not a PBT or vPvB
Glycine
No data
12.6 Other Adverse Effects
Methanol
None
Tris-Base
None
Glycine
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | Not Regulated | Not Regulated | Not Regulated | Not Regulated |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| Methanol | Yes | No | No | Yes | Yes |
| Tris-Base | No | No | No | Yes | No |
| Glycine | No | No | No | No | No |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
8/20/2013- Released Version 1.0
NFPA Codes
Health 2 Flammability 0 Reactivity 0
Dangers
Methanol
H225 - Highly flammable liquid and vapor.
H301 - Toxic if swallowed
H311 - Toxic in contact with skin.
H331 - Toxic if inhaled.
H371 - May cause damage to organs.
Tris-Base
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
Glycine
None
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.