Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEET
Conforms to regulation (EC) no. EU 453/2010
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
1.3 Details of the Supplier of the Safety Data Sheet
| Manufacturer National Diagnostics 305 Patton Drive Atlanta, GA 30036 (404) 699-2121 (800) 526-3867 info@nationaldiagnostics.com |
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H315 - Skin Corrosion/Irritation (Category 2)
H319 - Serious Eye Damage/Eye Irritation (Category 2A)
H335 - Specific Target Organ Toxicity, Single Exposure (Category 3)
2.2 Label Elements
| GHS Label Elements | ||
 | WARNING H315 - Causes skin irritation. | |
2.3 Other Hazards
None found.
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Solution of tris base and EDTA.
Component List
| Component | % Comp. | CAS # | EC # | 1278/2008 Classification |
|---|---|---|---|---|
| Ethylenediaminetetraacetic acid, disodium salt dihydrate | 4.7 | 6381-92-6 | 205-358-3 | H332 |
| Tris Hydrochloride | 15.3 | 1185-53-1 | 214-684-5 | H315, H319, H335 |
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
Ingestion
If swallowed, wash out mouth with water provided person is conscious. Call a physician.
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include coughing and shortness of breath.
Tris Hydrochloride:
Symptoms may include coughing and shortness of breath.
Ingestion
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
Tris Hydrochloride:
Symptoms may include irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
Skin
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation with redness and pain.
Tris Hydrochloride:
Symptoms may include irritation with redness and pain.
Eyes
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation, redness, pain, and corneal damage.
Tris Hydrochloride:
Symptoms may include irritation, redness, pain, and corneal damage.
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Water spray, dry chemical, alcohol-resistant foam, or carbon dioxide.
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
N.A.
Hazardous Decomposition Products
Nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide.
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
5.4 Further Information
No data available.
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
6.3 Methods and Materials for Containment and Cleaning Up
Use a vacuum cleaner equipped with charcoal exhaust scrubber or hose from mechanical exhaust ventilation system to vacuum spills. If this is not possible, sweep up spills and place in a covered waste disposal container. Flush area with water.
6.4 References to Other Sections
For disposal information see Section 13. For protective clothing and equipment see Section 8.
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Protect from physical damage. Isolate from incompatible materials (section 10).
Incompatibles
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Strong oxidizing agents, strong bases, aluminum, copper, copper alloys, nickel.
Tris Hydrochloride:
Bases, oxidizing agents, acids, aldehydes, copper, brass, and aluminum.
7.3 Specific End Uses
Investigational research by professional users
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: Ethylenediaminetetraacetic acid, disodium salt dihydrate
ACGIH Threshold Limit Value (TLV):
None Established
OSHA Permissable Exposure Limit (PEL):
None Established
Component: Tris Hydrochloride
ACGIH Threshold Limit Value (TLV):
None established
OSHA Permissable Exposure Limit (PEL):
None established
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator.
Eye Protection
Safety glasses.
Skin Protection
Wear protective gloves and clean body covering clothing.
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
| a. Appearance | Colorless liquid | b. Odor | Odorless |
| c. Odor Threshold | N.A. | d. pH | 9 |
| e. Melting/Freezing Point (oC) | ~6 | f. Boiling point (oC) | as water |
| g. Flash Point (oC) | N.A. | h. Evaporation Rate | water |
| i. Flammability | N.A. | j. Upper/Lower Flammability or Explosive Limits | N.A. |
| k. Vapor Pressure | water | l. Vapor Density (Air = 1) | Air |
| m. Relative Density | 1.12 | n. Water Solubility | miscible |
| o. Partition Coefficient n-octanol/water | Mixture | p. Autoignition Temperature (oC) | N.A. |
| q. Decomposition Temperature (oC) | Not applicable. | r. Viscosity | No data available. |
| s. Explosive Properties | N.A. | t. Oxidizing Properties | Not an oxidizer |
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Not reactive under recommended conditions of use and storage.
10.2 Chemical Stability
Stable at room temperature in closed containers under normal storage and handling conditions. Decarboxylates above 150C.
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
10.4 Conditions to Avoid
Incompatible materials, dust generation, excess heat.
10.5 Incompatible Materials
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Strong oxidizing agents, strong bases, aluminum, copper, copper alloys, nickel.
Tris Hydrochloride:
Bases, oxidizing agents, acids, aldehydes, copper, brass, and aluminum.
10.6 Hazardous Decomposition Products
Nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide.
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
12760
Dermal Rabbit LD50 (mg/kg)
N.D.
Component Cancer List Status
| NTP Carcinogen | |||
| Known | Anticipated | IARC Category | |
|---|---|---|---|
| Ethylenediaminetetraacetic acid, disodium salt dihydrate | No | No | None |
| Tris Hydrochloride | No | No | None |
Potential Health Effects
Inhalation
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes irritation of the mucous membrane and upper respiratory tract.
Tris Hydrochloride
May cause respiratory tract irritation. The toxigological properties of this substance have not been fully investigated.
Ingestion
Ethylenediaminetetraacetic acid, disodium salt dihydrate
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. Exposure may cause kidney injury, muscle cramps, bone-marrow depression, and a generalized allergic reaction.
Tris Hydrochloride
May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause severe gastrointestinal tract irritation with nausea, vomiting and possible burns. The toxicological properties of this substance have not been fully investigated.
Skin
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes skin irritation. Causes redness and pain.
Tris Hydrochloride
Causes skin irritation. Causes redness and pain.
Eyes
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes eye irritation. Causes redness and pain.
Tris Hydrochloride
Causes eye irritation. Causes redness and pain.
Carcinogenicity
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Not listed by NTP or IARC as a known or anticipated carcinogen.
Tris Hydrochloride
Not listed by OSHA, ACGIH, NIOSH, NTP or IARC as a known or anticipated carcinogen.
Mutagenicity
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Cytogenetic Analysis: intraperitoneal - mouse 50mmol/L. DNA Inhibition: hamster fibroblast 500ug/L, rabbit kidney 250 umol/L.
Tris Hydrochloride
No information available.
Reproductive Toxicity
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Fertility: Post-implantation mortality, oral - rat TDLo = 7632 mg/kg.
Tris Hydrochloride
No information available.
Teratogenic Effects
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Embryo or Fetus: Stunted fetus, oral - rat TDLo = 7632 mg/kg. Specific developmental abnormalities: cardiovascular, craniofacial, musculoskeletal, respiratory, and urogenital, oral - rat TDLo = 7632 mg/kg.
Tris Hydrochloride
No information available.
Routes of Entry
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Inhalation, ingestion or skin contact.
Tris Hydrochloride
Inhalation, ingestion or skin contact.
Target Organ Statement
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No information available
Tris Hydrochloride
No information available
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: Ethylenediaminetetraacetic acid, disodium salt dihydrate
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | LC50 (96hr, Lepomis macrochirus ):41mg/L | EC50 (485hr daphnia) 140mg/L | EC 50 (72hrs)>100mg/L | EC50>500mg/L |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | LOEC (Nicotiana tabacum ) 420mg/L | No data |
COMPONENT: Tris Hydrochloride
| Vertebrates | Invertebrates | Algae | Microorganisms | |
|---|---|---|---|---|
Aquatic Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
| Birds | Arthropods | Plants | Microorganisms | |
|---|---|---|---|---|
Terrestrial Environment Toxicity (ppm unless otherwise noted) | No data | No data | No data | No data |
12.2 Persistence and Degradability
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Biodegradable (55% degradation in 20 days)
Tris Hydrochloride
No data
12.3 Bioaccumulative Potential
Ethylenediaminetetraacetic acid, disodium salt dihydrate
BCF 1.1-1.8
Tris Hydrochloride
No data
12.4 Mobility in Soil
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No data
Tris Hydrochloride
No data
12.5 Results of PBT and vPvB Assessment
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No data
Tris Hydrochloride
No data
12.6 Other Adverse Effects
Ethylenediaminetetraacetic acid, disodium salt dihydrate
None
Tris Hydrochloride
None
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
SECTION 14 - TRANSPORT INFORMATION
| ADR/RID | IATA | IMO | DOT | |
|---|---|---|---|---|
| 14.1 UN Number | N.A. | N.A. | N.A. | N.A. |
| 14.2 Shipping Name | Not Regulated. | Not Regulated. | Not Regulated. | Not Regulated. |
| 14.3 Hazard Class | N.A. | N.A. | N.A. | N.A. |
| 14.4 Packing Group | N.A. | N.A. | N.A. | N.A. |
| 14.5 Environmental Hazards | N.A. | N.A. | Not regulated | N.A. |
| 14.6 Special Precautions | N.A. | N.A. | N.A. | N.A. |
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
SARA 311/312 Hazard Categories
| Component | Fire | Pressure | Reactivity | Acute | Chronic |
|---|---|---|---|---|---|
| Ethylenediaminetetraacetic acid, disodium salt dihydrate | No | No | No | Yes | No |
| Tris Hydrochloride | No | No | No | Yes | No |
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
10/25/2013 - Released Version 1.0
NFPA Codes
Health 1 Flammability 0 Reactivity 0
Dangers
Ethylenediaminetetraacetic acid, disodium salt dihydrate
H332 - Harmful if inhaled.
Tris Hydrochloride
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.