Oxygen Assay Protocols

Hydrogen Peroxide Assay Protocol

Assay Reagent Preparation
Prepare 1.8ml of reagent per sample to run tests in duplicate.
1. To prepare 20ml: Combine 19.8ml of Component A with 0.2ml
Component B.
2. The mixture is stable at room temperature for one working day.

Assay Procedure
1. Mix 0.9ml of Assay Reagent with up to 0.1ml of sample.
2. Incubate at room temperature for at least 30 minutes to allow
for complete color development.
3. Read absorbance at 560nm.


Diogenes Protocol

 Solution Preparation
1. Add 1.0 ml of deionized water to the Diogenes Reagent that is
contained in the vial. Mix until completely dissolved.
2. Add 9.0 ml of deionized water to the Diogenes Activator that is
contained in the bottle. Mix until completely dissolved.
3. Add the contents in the vial (Diogenes Reagent) to the contents in
the bottle (Diogenes Activator), recap the bottle and shake vigorously
to mix.
4. The combined solution (10 ml) comprises the Diogenes Complete

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