Radioactive decay occurs with the emission of particles or electromagnetic radiation from an atom due to a change within its nucleus.
Most research applications of radioisotopes require an eventual quantitation of the isotope, which is done by measuring the intensity of radiation
By eliminating the combustion steps needed for gas phase analysis, the introduction of liquid scintillation counting (LSC) reduced the time require
A beta particle, passing through a scintillation cocktail, leaves a trail of energized solvent molecules.
While the effectiveness of a scintillation cocktail may be expressed a number of ways, it is most often given as the percentage of emission events
Living creatures contain both hydrophobic and hydrophilic compounds, any of which may be labeled during the course of a radioactive experiment.
Another commonly encountered artifact is chemiluminescence.
An aspect of LSC which must be considered in experimental design, is waste disposal.
Liquid scintillation counting of discrete samples is conceptually straightforward.
In a typical TLC experiment, the radioactivity is detected at two points: after TLC it is analyzed by autoradiography
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