- Rinse fixed tissue in running water for five minutes.
- Submerge the tissue sample in a volume of CalciClear equal to at least ten times the volume of the sample. Take care that the CalciClear has free access to all surfaces of the tissue.
- Check tissue every thirty to sixty minutes. Although some workers consider decalcification complete when the tissue begins to float, for some types of tissue this is not an accurate indicator of the endpoint of decalcification. A chemical test may be employed for to more accurately confirm the endpoint of decalcification.
- Changing the solution is recommended every two or three hours.
Full Protocol Sheet: