Histology Articles

Embedding

For mechanical support during the sectioning process, tissue must be infiltrated with an embedding medium.

Sectioning

Once embedded, tissues are cut into thin sections ready to be placed on a slide.

Artifacts in Histologic Sections

Artifacts that appear in stained slides may result from a number of causes including improper fixation, the type of fixative, poor dehydration, imp

The Chemistry of Dyes and Staining

Histological staining involves the use of dyes to highlight specific intra- or extracellular elements within tissue.

Staining Procedures

Most dyes used to visualize the membranes and organelles of the cell are water soluble.

Mounting Tissue Sections

To preserve and support a stained section for light microscopy, it is mounted on a clear glass slide, and covered with a thin glass coverslip.

Immunohistochemistry

Immunohistochemistry is the application of antibody/antigen interactions to provide information about biological systems.

Detection Systems in Immunohistochemistry

Light microscopy makes use of primarily two detection systems for immunohistochemistry - fluorescence and enzyme labeling, while electron microscop

Antibody Binding

The antibody systems used in Immunohistochemistry can be broadly divided into two types, direct and indirect.

Electron Microscopy

The resolution of a microscope is limited by the wavelength of light passing through the sample.

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