For mechanical support during the sectioning process, tissue must be infiltrated with an embedding medium.
Once embedded, tissues are cut into thin sections ready to be placed on a slide.
Artifacts that appear in stained slides may result from a number of causes including improper fixation, the type of fixative, poor dehydration, imp
Histological staining involves the use of dyes to highlight specific intra- or extracellular elements within tissue.
Most dyes used to visualize the membranes and organelles of the cell are water soluble.
To preserve and support a stained section for light microscopy, it is mounted on a clear glass slide, and covered with a thin glass coverslip.
Immunohistochemistry is the application of antibody/antigen interactions to provide information about biological systems.
Light microscopy makes use of primarily two detection systems for immunohistochemistry - fluorescence and enzyme labeling, while electron microsco
The antibody systems used in Immunohistochemistry can be broadly divided into two types, direct and indirect.
The resolution of a microscope is limited by the wavelength of light passing through the sample.
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