Histology Articles

Overview of Fixation

To maintain the tissue in as lifelike a state as possible, tissue for analysis is usually placed directly into a fixative solution upon removal fr

Aldehyde Fixatives

Formaldehyde and glutaraldehyde are the most commonly used aldehyde fixatives.

Non-Aldehyde Fixatives

Mercury Based Fixatives
 

Factors Affecting Fixation

Fixation protocols are usually straightforward.

Working Safely with Fixatives

Fixatives are among the most hazardous substances used in life science research.

Decalcifying Tissue for Histological Processing

The removal of calcium deposits is essential for good embedding procedure.

Overview of the Paraffin Technique

Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope.

Suggested procedures for processing fixed tissue

Here are a few guidelines which you can use to process fixed tissue using Histo-Clear and Histo-Clear II:

Dehydration

Dehydration is usually carried out by transferring the tissue through solutions of increasing alcohol concentration, until 100% alcohol is reached

Clearing Tissue Sections

The step following dehydration is called "clearing" and consists of replacing the dehydrant with a substance that will be miscible with the embeddi

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