To maintain the tissue in as lifelike a state as possible, tissue for analysis is usually placed directly into a fixative solution upon removal fr
Formaldehyde and glutaraldehyde are the most commonly used aldehyde fixatives.
Fixation protocols are usually straightforward.
Fixatives are among the most hazardous substances used in life science research.
The removal of calcium deposits is essential for good embedding procedure.
Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope.
Here are a few guidelines which you can use to process fixed tissue using Histo-Clear and Histo-Clear II:
Dehydration is usually carried out by transferring the tissue through solutions of increasing alcohol concentration, until 100% alcohol is reached
The step following dehydration is called "clearing" and consists of replacing the dehydrant with a substance that will be miscible with the embeddi
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