UreaGel 6 and 8 Protocol

Mix Solutions
Add appropriate volumes of SequaGel 6 or 8 Monomer Solution and SequaGel Complete Buffer to a thick-walled Erlenmeyer flask (see Table 1). If desired, the solution may be degassed by stirring under vacuum for two minutes.
Bring to room temperature before polymerization.

Table 1: Volumes of SequaGel 6 or 8 Monomer Solution and
SequaGel Complete Buffer to prepare 100mL gel solution

SequaGel Complete
Buffer
SequaGel Monomer
Solution
10%
Ammonium Persulfate
20 mL 80 mL 800 μL

 


Add APS and Cast Gel
Add FRESHLY PREPARED 10% Ammonium Persulfate (see Table 1). Swirl gently to mix, and cast the gel. Insert the comb and allow to polymerize one to two hours. NOTE: After two hours of polymerization wrap each end of the gel cassette with clear plastic wrap. This is important to keep the ends of the gel from drying and to maintain sample well integrity. Appropriately wrapped gels may be stored for up to 48 hours.
 

 

Catalog Number: 
EC-836
EC-838
Full Protocol Sheet: 

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