Mix UreaGel System Components:
Determine how much UreaGel 29:1 Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix.

For 100 ml Gel Casting Solution:

V_c = (V_t) (X)/25
V_b = 0.1 (V_t)
V_d = V_t - (V_c + V_b)

where:

V_c = UreaGel 29:1 Concentrate Volume
V_b = UreaGel Buffer Volume
V_d = UreaGel Diluent Volume
V_t = Total Casting Solution Volume
X = % Gel Desired

EXAMPLE:

To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows:
V_c =(100) (8)/25 = 32ml UreaGel 29:1 Concentrate
V_b = 0.1 (100) = 10ml UreaGel Buffer
V_d = 100 - (32 + 10) = 58ml UreaGel Diluent

Add Initiators and Cast Gel:
Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.