ProtoGel Quick Cast Protocol
Twenty Minute Casting
ProtoGel Quick-Cast contains the monomers and buffer components to produce a 12% gel.
- Measure out the volume of ProtoGel Quick-Cast needed to fill the cassette - typically 10ml for one mini-gel, 15ml for two.
- Add 100 microliters of fresh 10% APS and 10 microliters of TEMED per 10ml ProtoGel Quick-Cast. Mix briefly and pour into the gel cassette.
- 3. Insert comb and allow to polymerize at room temperature for 20 minutes. The gel is now ready to run.
For best results, it is recommended you use ProtoGel Quick-Cast Loading Buffer. Simply mix your samples with an equal volume of ProtoGel Quick-Cast Loading Buffer, load and run.
Catalog Number:
EC-895
Full Protocol Sheet: