NucliStain Protocol

  1. To prepare a working strength solution of NucliStain, add one part NucliStain concentrate to one-hundred parts of distilled water. Mix until the solution is uniform. Prepare sufficient stain solution to fully submerge the gel.
  2. Immerse the gel in the stain solution for 20–30 minutes.
  3. Immerse the gel in distilled water for 3–4 hours to destain the blue background. Bands can often be visualized minutes after destaining. However, for increased sensitivity, overnight destaining is recommended.
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