Glass Bond Protocol
Glass Bond Method of Use
1. Glass plates should be clean and dry before starting the bonding procedure (distilled water should always be used as a final rinse).
2. Prepare enough bonding solution to fully submerge all the plates that are to be treated. Take 500 ml of water and adjust the pH to 3.5 by adding glacial acetic acid (~50μl). Add 2.0 ml of Glass Bond to the water and mix well with a magnetic stirrer until the liquid dissolves (~20 minutes).
3. Pour the Glass Bond solution into a suitable vessel containing the clean electrophoresis plates Incubate for 1-3 hours with stirring.
4. Remove plates from the incubation vessel and rinse them with distilled water. Allow the plates to air dry and wipe them gently with a non-scratch tissue.
5. Store the plates interleaved between sheets of dry paper in a plastic storage bag.
6. Acrylamide gels denatured with urea will crack upon drying unless the urea is removed. To remove the urea, fix the gels for 30 minutes in 10% acetic acid-10% methanol.
7. If it is necessary to remove the gels intact from the plate, submerge the plate with the bonded gel in water and carefully run a razor between the gel and glass surface.
For more details, please see the full protocol linked below.
Full Protocol Sheet: