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October 4, 2013

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

Glass Bond Protocol

Glass Bond Method of Use
Basic Protocol
1. Glass plates should be clean and dry before starting the bonding procedure (distilled water should always be used as a final rinse).
 
2. Prepare enough bonding solution to fully submerge all the plates that are to be treated. Take 500 ml of water and adjust the pH to 3.5 by adding glacial acetic acid (~50μl). Add 2.0 ml of Glass Bond to the water and mix well with a magnetic stirrer until the liquid dissolves (~20 minutes).
 
3. Pour the Glass Bond solution into a suitable vessel containing the clean electrophoresis plates  Incubate for 1-3 hours with stirring.
 
4. Remove plates from the incubation vessel and rinse them with distilled water. Allow the plates to air dry and wipe them gently with a non-scratch tissue.
 
5. Store the plates interleaved between sheets of dry paper in a plastic storage bag.
 
6. Acrylamide gels denatured with urea will crack upon drying unless the urea is removed. To remove the urea, fix the gels for 30 minutes in 10% acetic acid-10% methanol.
 
7. If it is necessary to remove the gels intact from the plate, submerge the plate with the bonded gel in water and carefully run a razor between the gel and glass surface. 
 
 
For more details, please see the full protocol linked below.
Catalog Number: 
EC-620
Full Protocol Sheet: 

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