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Electrophoresis

AquaPor HR GTAC Agarose

$121.00$295.00

Catalog Number: EC-205
Quantity discount when you buy 4 or more 100g units!
  • High Strength High-Resolution Agarose
  • Excellent for small DNA fragments, PCR products, and proteins
  • Ultra-Fine particle size for fastest dissolution

Description

Quantity discount when you buy 4 or more 100g units!
  • High Strength High-Resolution Agarose
  • Excellent for small DNA fragments, PCR products, and proteins
  • Ultra-Fine particle size for fastest dissolution

AquaPor HR GTAC is our highest resolving agarose, specifically manufactured for the optimal electrophoretic separation of small DNA fragments, PCR products, and proteins. AquaPor HR will resolve DNA down to 2% size difference. Uniform particle size minimizes boilover. Additionally, the high quality of this agarose has allowed the certification of AquaPor HR for Taq DNA-Polymerase directed PCR (re)amplification directly in the presence of the remelted gel. AquaPor HR is GTAC certified to be DNase, RNase, and protease free, and certified for in-gel PCR.

EEO (-mr) Gel Strength (g/cm2) Gel Temp (°C)
≤ 0.12 ≥ 400 @ 1.5% ≤ 26 @ 1.5%
Melt Temp (°C) Sulfate (%) DNase, RNase, Protease
≤ 80 @ 1.5% ≤ 0.20 None

Additional information

Weight 0.2 lbs
Dimensions 6 × 3 × 3 in

Protocol

DISSOLVING AQUAPOR AGAROSES

  1. Add room temperature buffer to a flask that is 2.5 – 4 times the volume of gel solution. Add a teflon-coated stir bar.
  2. Add AquaPor powder while stirring vigorously so the agarose is dispersed uniformly. Stir for 2 minutes to hydrate the agarose.
  3. Tare the flask and solution.
  4. Place in a microwave oven and heat at 100% power using 20 – 60 second intervals. Swirl gently between intervals to resuspend the agarose.
  5. Continue the cycle of heating and swirling until the agarose is completely dissolved (no visible particles are present).
  6. Add distilled water to obtain the initial weight and mix.
  7. Cool the solution to 50 – 60°C before pouring the gel.

* Chill the gel for 30 minutes prior to comb removal when using AquaPor LM, HR, and low (<1%) concentration of AquaPor LE and ES.

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Safety Overview

Appearance and Odor
White powder

EMERGENCY OVERVIEW – IMMEDIATE HAZARD

AS PART OF GOOD INDUSTRIAL AND PERSONAL HYGIENE AND SAFETY PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.

Full SDS

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