Electrophoresis Articles

Sample Preparation for Native Protein Electrophoresis

Samples to be run on native gels should be prepared in a way which minimizes denaturation of the proteins.

Gel Preparation for Native Protein Electrophoresis

The basic protocols for preparing Native PAGE gels is the same as for discontinuous SDS PAGE gels, substitut

Activity Stains

Samples to be run on native gels should be prepared in a way which minimizes denaturation of the proteins.

Immuno-Electrophoresis / Immuno-Diffusion

Antibodies are produced by the immune system in response to foreign macromolecules.

Isoelectric Focusing

Conventional electrophoresis techniques can separate up to 100 different proteins on one run.

UV Shadowing

Detection of DNA/RNA in solution is generally done by measuring its UV absorbance at 260 nm.

Post-Electrophoretic Visualization with Nuclistain

National Diagnostics' Nuclistain offers a significant increase in sensitivity over UV shadowing,

Ethidium Bromide Staining

The most commonly used stain for detecting DNA/RNA is ethidium bromide.

Silver Staining DNA Gels

Silver staining is more sensitive than ethidium bromide for double stranded DNA, and detects single stranded DNA or RNA with no loss in sensitivity

An Overview of Northern and Southern Blotting

A DNA or RNA probe will selectively hybridize with nucleic acid molecules of complementary sequence in a sample.

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