Electrophoresis Articles

Agarose Gel Electrophoresis of DNA and RNA - Uses and Variations

Agarose electrophoresis is used for a variety of purposes. Specialty grades of agarose have been developed to fulfill specific requirements.

Preparation of Agarose Gels

Use the tables below corresponding to the AquaPor agarose of choice to determine the percentage gel to cast.

Restriction Digest Mapping

Restriction endonucleases are enzymes that cleave double stranded DNA at specific sites, generally 4, 6 or 8 base palindromic sequences.

DNA/RNA Purification from Agarose Gels - Electroelution

The most popular alternative to glass powder elution for the complete purification of DNA from agarose is electroelution.

In Gel Enzyme Reactions

In many cases, the processing of DNA by enzymes is not impeded by agarose.

RNA Electrophoresis

Electrophoretic analysis of RNA presents unique challenges. RNA is isolated in single stranded form, without complementary sequences.

PFGE and FIGE

Standard agarose gels can resolve DNA fragments up to 75 kb. Often, analysis of genomic DNA requires resolution of megabase (mb) fragments.

Denaturing Protein Electrophoresis: SDS-PAGE

In their native form, proteins fold into a variety of shapes, some compact, some elongated.

Peptide Mapping

Peptide mapping involves controlled cleavage of a pure protein with small amounts of a pure protease to generate peptides of characteristic, repro

Native Protein Electrophoresis

Proteins run on PAGE in the absence of SDS will separate on the basis of their charge to mass

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