Electrophoresis Articles

PCR Analysis: An Examination

The polymerase chain reaction (PCR) is a powerful technique which uses repetitive cycles of primer annealing, primer extension, and product denatur

Gel Preparation for Native PAGE of DNA

Native PAGE gels are prepared by mixing an acrylamide/bisacrylamide monomer concentrate (AccuGel

PCR Analysis: Yield and Kinetics

PCR reactions produce product in a nonlinear pattern (See figure below).

Gel Electrophoresis of PCR Products

Gel electrophoresis of PCR products is the standard method for analyzing reaction quality and yield.

Mobility Shift Assay

Protein bound to a small piece of DNA will alter the electrophoretic mobility of that DNA fragment.

DNA/RNA Purification from PAGE Gels

Polyacrylamide gel electrophoresis yields individual bands of extremely high purity, in that only one nucleic acid sequence tends to be present in

Conformational Analysis

Native DNA PAGE gels can be used to detect small mutational differences between DNA molecules.

Heteroduplex Analysis

Double-stranded DNA is not a completely straight rigid rod.

SSCP Analysis

Single-stranded DNA can adopt multiple conformations under non-denaturing conditions.

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