Electrophoresis Articles

Using PAGE to Determine Nucleic Acid Molecular Weight

Molecular weight determination is the most basic use of denaturing polyacrylamide gel electrophoresis.

Measuring Molecular Weight with SDS-PAGE

The mobility (Rf) of a molecule in gel electrophoresis is determined by its free solution mobility, Y0 (= mobility in a gel o

Manual Sequencing

DNA sequences are determined by a two step process.

Maxam & Gilbert Sequencing

There are four chemical cleavage reactions at the core of the Maxam and Gilbert sequencing system.

Sanger Sequencing

In Sanger dideoxy terminator sequencing, the sample DNA is used as a template for a DNA polymerase.

Enzyme Linked Immunosorbent Assay (ELISA)

One of the most straightforward applications of immunological detection is the ELISA, or enzyme linked immunosorbent assay.

Overview of Western Blotting

Proteins can also be detected immunologically following electrophoresis, a technique known as Western blotting.

Automated Sequencers

Automated sequencing systems make use of fluorescent dye labeling, in combination with laser scanning and computerized data acquisition and process

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