Electrophoresis Articles

Horizontal and Vertical Gel Systems - The Horizontal Gel System

A gel electrophoresis apparatus must allow the researcher to maintain a uniform electric field across the gel, provide cooling to prevent thermal a

Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA

The electrophoretic analysis of single stranded nucleic acids is complicated by the secondary structures assumed by these molecules.

Sample Prep for Denaturing PAGE of DNA

DNA samples for denaturing gel electrophoresis must be denatured prior to loading, to avoid time dependent denaturation artifacts on the gel.

Sample Preparation for SDS-PAGE

SDS is a powerful detergent, which will solubilize many cells and tissues.

Preparing Denaturing DNA & RNA Gels

Sequencing gels are poured between two glass plates separated by spacers. The spacers are typically no more than 0.2mm in thickness.

Gel Preparation for SDS-PAGE

Two categories of buffer systems are available for SDS PAGE: continuous and

Casting Gradient Gels

Gradient gels are cast with a higher concentration of acrylamide at the bottom than the top.

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