Electrophoresis Articles

The Mechanical and Electrical Dynamics of Gel Electrophoresis -- Electrophoresis System Dynamics

Electrophoresis System Dynamics

The Electrophoresis Matrix

In gel electrophoresis the matrix forces sample components to separate by size, as they move through its porous structure.

The Polyacrylamide Matrix

Polyacrylamide gels are formed by the polymerization of acrylamide in aqueous solution in the presence of small amounts of a bifunctional crosslinker. The crosslinker is usually methylenebisacrylamide (bis, or MBA).

The Agarose Matrix

A natural colloid extracted from seaweed, agarose is a linear polysaccharide made up of the repeating unit agarobiose, which consists of alternatin

Electrophoresis Buffers--The Henderson-Hasselbalch Equation

In its simplest form, a buffered solution contains a mixture of a weak acid and its conjugate base.

Electrophoresis Buffers-Choosing the Right Buffer

Several factors to consider when choosing a buffer include:

Homogeneous Buffer Systems

In a homogeneous buffer system, the identity and concentration of buffer components are the same in the gel and the tanks.

Multiphasic Buffer Systems

Employing gel and buffer discontinuities to produce sharp separation among sample components, multiphasic electrophoresis design can improve the re

Isotachophoresis

Isotachophoresis is a method of electrophoresis which employs the basic principles of the stacking gel phase of multiphasic systems discussed in th

Buffer Additives-Surfactants

A crucial initial step in the electrophoretic separation of proteins is the solubilization of the sample molecules.

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