Electrophoresis Articles

Mechanism of Immunostaining

The highly specific binding interaction between antibodies and their unique antigens has been exploited to create sensitive and specific detection

Gel Electrophoresis of RNA & Post Electrophoretic Analysis

Agarose electrophoresis of RNA requires the inclusion of denaturing agents in the gel.

Preparation of Denaturing Agarose Gels

A variety of denaturants can be used with agarose.

Buffer Additives-Reducing Agents

Disulfide bonds between or within sample protein molecules can lead to the formation of aggregates as well as play a role in the binding of the sub

Buffer Additives-Hydrogen Bonding Agents

In most forms of electrophoresis the solution perfusing the gel matrix typically contains one or more substances in addition to the buffer salts.

The Polyacrylamide Matrix-Buffer Strength

The buffer system in electrophoresis controls the pH of the gel, preventing damage to sample molecules and, in certain cases, controlling the ioniz

Biological Macromolecules: Nucleic Acids

Nucleic Acids
 

Biological Macromolecules - Proteins

Proteins

The Mechanical and Electrical Dynamics of Gel Electrophoresis - Intro and Sample Mobility

The term "electrophoresis" refers simply to the movement of particles by an electric force.

The Mechanical and Electrical Dynamics of Gel Electrophoresis - Ohm's Law

Ohm's Law: Relationships between electrical parameters

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