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October 4, 2013

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

Buffer Additives-Reducing Agents

Disulfide bonds between or within sample protein molecules can lead to the formation of aggregates as well as play a role in the binding of the subunits of many proteins. It is usually desirable to cleave disulfide linkages prior to the protein electrophoresis. For this reason, disulfide bond reducing agents, such as 2-mercaptoethanol or dithiothreitol, are typically present in sample buffers. These substances can also be added to the cathode tank. However, 2-mercaptoethanol or dithiothreitol are typically not added to the gel casting solution because their presence inhibits gelation.

2-MercaptoethanolDithiothreitol
2-Mercaptoethanol
Dithiothreitol

 

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