- Staining Occurs During the Run
- Ideal Prep for Mass Spec
- 10ng Sensitivity in 30 Minutes!
 The Insite System is an in-gel fluorescent stain for proteins, providing excellent sensitivity (10ng) more quickly than any other detection method. The Insite stain runs with the proteins during electrophoresis. No staining step is required after electrophoresis. To use the Insite System, researchers simply mix Insite Buffer Concentrate with Insite Dye Concentrate and QS with deionized water to formulate their cathode tank buffer for SDS-PAGE. The Insite dye runs with the proteins during electrophoresis. After a short destain in water following electrophoresis, the bands are visualized under UV transillumination.
Proteins stained with the Insite System may be easily and reproducibly recovered at high yield from SDS-PAGE gels for analysis by mass spectroscopy.
The image on the left above shows the mass spec of Carbonic Anhydrase. Above right, the image shows the mass spec of Carbonic Anhydrase that has been electrophoresed by SDS-PAGE, visualized by the Insite System, and Purified from the Gel Slice by Crush and Soak Technique.
The Insite System contains sufficient material to stain 60 mini-gels.
Catalog Number: EC-759
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