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ProtoGel Sample Prep Kit
ProtoGel Sample Prep Kit

  • Patented system for SDS-PAGE sample prep
  • Removes interfering contaminants
  • Concentrates dilute samples
  • Prevents gel failures
  • Simple and inexpensive



Unpurified and purified samples in SDS-PAGE
Purification
Contaminants in the sample such as high salt or urea lead to blurred bands or smiling gels in SDS-PAGE. With the ProtoGel Sample Prep Kit, interfering substances from upstream applications can no longer gain entry to the well. Contaminants are washed away with a simple method. The sample loaded contains only pure protein and loading buffer with no contaminants remaining to impede reproducible, high-resolution results.

Lanes 1 and 3 are unconcentrated. Lanes 2 and 4 are concentrated.
Concentration
In addition to purification, proteins previously too dilute for SDS-PAGE can now be concentrated prior to electrophoresis with a simple method. The ProtoGel Sample Prep Kit concentrates proteins as dilute as 25ng/100µl. The unique ProtoGel Sample Prep Kit casts the finest net of any recovery system, concentrating all proteins in high yield regardless of identity. With the ProtoGel Sample Prep Kit, the purity and concentration of your SDS-PAGE samples are both under your control.

Catalog Number: EC-884
Format:
Download Protocol



Material Safety Data Sheets


ProtoGel Sample Prep Kit - Reagent A     HTML        PDF

ProtoGel Sample Prep Kit - Reagent B     HTML        PDF

Protein Loading Buffer Blue 2X     HTML        PDF

Qty:
Related Products   
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Ready-to-use gel solution for SDS-PAGE that combines the convenience of pre-cast gels with the economy of casting your own.
 
ProtoGel (30%)
30% concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
ProtoGel (40%)
Concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
AccuGel 29:1
Concentrated solution of acrylamide and bis-acrylamide (29:1) for native DNA electrophoresis and SDS-PAGE. Filtered, deionized, and stabilized.
 
5X Protein Loading Buffer
Reducing sample buffer for SDS-polyacrylamide gel electrophoresis.
 
Tris-Glycine-SDS PAGE Buffer (10X)
Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration.
 
ProtoGel Resolving Buffer (4X)
4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE.
 
ProtoGel Stacking Buffer
4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Tris-Tricine-SDS PAGE Buffer (10X)
Alternative tank buffer for the SDS-PAGE separation of small proteins.
 
ProtoBlue Safe
Eco-friendly, ultra-sensitive colloidal coomassie G-250 stain.
 
ProtoMarkers
Broad range pre-stained protein markers for SDS-PAGE.
 
Ammonium Persulfate - ULTRA PURE
Exceeds ACS standards. Low absorbed water results in consistent initiation.
 
TEMED - ULTRA PURE
Fractionally distilled to remove all trace metals and amine impurities. Stored under nitrogen.
 

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CURRENCIES

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