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ProtoGel (30%)
ProtoGel (30%)

  • 37.5:1 Acrylamide to Bisacrylamide Stabilized Solution
  • Optimized for SDS-PAGE of Proteins (Laemmli gels)
  • Consistently Crystal Clear Gels, Zero Fluorescence
  • Stabilized for Long Shelf Life
National Diagnostics ProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed. ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.
Storage: ProtoGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20°C).

Catalog Number: EC-890
Size:
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APPLICATIONS OF PROTOGEL (30%)
The Polyacrylamide Matrix
Denaturing Protein Electrophoresis: SDS-PAGE
Gel Preparation for SDS-PAGE
Casting Gradient Gels
Protein Purification using SDS-PAGE
Native Protein Electrophoresis
Gel Preparation for Native Protein Electrophoresis
Isoelectric Focusing
Method for Western Blotting
Measuring Molecular Weight with SDS-PAGE
Peptide Mapping
Related Products   
ProtoGel Sample Prep Kit
The ProtoGel Sample Prep Kit represents a convenient, universal method for concentrating and optimizing protein samples prior to SDS-PAGE. Patent pending.
 
ProtoGel Quick-Cast 12%
Ready-to-use gel solution for SDS-PAGE that combines the convenience of pre-cast gels with the economy of casting your own.
 
ProtoGel (40%)
Concentrated solution of acrylamide and bis-acrylamide, 37.5 : 1 ratio. Filtered, deionized, and stabilized.
 
AccuGel 29:1
Concentrated solution of acrylamide and bis-acrylamide (29:1) for native DNA electrophoresis and SDS-PAGE. Filtered, deionized, and stabilized.
 
5X Protein Loading Buffer
Reducing sample buffer for SDS-polyacrylamide gel electrophoresis.
 
Tris-Glycine-SDS PAGE Buffer (10X)
Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration.
 
ProtoGel Resolving Buffer (4X)
4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE.
 
ProtoGel Stacking Buffer
4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Protein Loading Buffer Blue (2X)
Ready to use sample buffer for SDS-PAGE.
 
Tris-Tricine-SDS PAGE Buffer (10X)
Alternative tank buffer for the SDS-PAGE separation of small proteins.
 
ProtoBlue Safe
Eco-friendly, ultra-sensitive colloidal coomassie G-250 stain.
 
ProtoMarkers
Broad range pre-stained protein markers for SDS-PAGE.
 
Ammonium Persulfate - ULTRA PURE
Exceeds ACS standards. Low absorbed water results in consistent initiation.
 
TEMED - ULTRA PURE
Fractionally distilled to remove all trace metals and amine impurities. Stored under nitrogen.
 

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CURRENCIES

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