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SequaGel - UreaGel System
SequaGel - UreaGel System

  • Casts 19:1 Denaturing Gels up to 20% Monomer
  • Certified RNase and DNase Free
  • Consistently Crystal Clear Gels
  • Twelve Month Shelf Life at Room Temperature
The UreaGel System consists of UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the UreaGel System the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 Acrylamide/Bisacrylamide). Liter bottles of UreaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution. UreaGel Diluent is supplied in 450 ml and 1 liter bottles containing 7.5M urea in deionized water. UreaGel Buffer is supplied in 100 ml and 200 ml bottles containing 0.89M Tris-Borate-20mM EDTA buffer pH 8.3 (10X TBE) and 7.5M urea.
Storage: UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer are stable for one year when stored tightly capped, in a dark area at room temperature (20°C).

Catalog Number: EC-833
Component(s):
Download Protocol

Reference Articles

Material Safety Data Sheets


UreaGel Concentrate     HTML        PDF

UreaGel Buffer     HTML        PDF

UreaGel Diluent     HTML        PDF

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APPLICATIONS OF SEQUAGEL - UREAGEL SYSTEM
Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA
Preparing Denaturing DNA & RNA Gels
Manual Sequencing
Pouring Sequencing Gels
RNA Mapping
S1 Mapping
Ribonuclease Protection
Primer Extension
DNase I Footprinting
In-situ DNase Footprinting
Methylation & Uracil Interference Assays
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