Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEETNational Diagnostics
Conforms to regulation (EC) no. EU 453/2010
 
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
Product Name: Methylene Blue Product Number: HS-525
 
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
 
1.3 Details of the Supplier of the Safety Data Sheet
Manufacturer
National Diagnostics
305 Patton Drive
Atlanta, GA 30036
(404) 699-2121
(800) 526-3867
info@nationaldiagnostics.com
 
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
 
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H302 - Acute Toxicity-Oral (Category 4)
 
2.2 Label Elements
GHS LABEL ELEMENTS AND CLASSIFICATION
GHS Label Elements
Harmful 
WARNING
H302 - Harmful if swallowed
P264 - Wash skin thoroughly after handling.
P301+P312 - IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician IF you feel unwell.
P305+P351+P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
  
  
 
2.3 Other Hazards
None found.
 
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.1 Substance
Chemical Names/Description
Basic Blue 9; Methelyne blue trihydrate; 3,7-Bis(dimethylamino)phenazathionium chloride trihydrate
 
Chemical Formula
C16H18ClN3S*xH2O
 
Component List
Component % Comp. CAS # EC #
METHYLENE BLUE>95 61-73-4  200-515-2 
 
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
 
Ingestion
If swallowed, wash out mouth with water provided person is conscious. Call a physician.
 
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
 
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
 
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Causes respiratory tract irritation.
 
Ingestion
Nausea, vomiting, diarrhea, and gastritis.
 
Skin
Causes skin irritation.
 
Eyes
Causes eye irritation and possible injury.
 
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
 
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Water spray, dry chemical, alcohol-resistant foam, or carbon dioxide.
 
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Highly toxic gases may be involved in fires of this product.
 
Hazardous Decomposition Products
Hydrogen chloride, nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, sulfur oxides, including sulfur oxide and sufur dioxide.
 
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
 
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
 
5.4 Further Information
No data available.
 
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
 
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
 
6.3 Methods and Materials for Containment and Cleaning Up
Use a vacuum cleaner equipped with charcoal exhaust scrubber or hose from mechanical exhaust ventilation system to vacuum spills. If this is not possible, sweep up spills and place in a covered waste disposal container. Flush area with water.
 
6.4 References to Other Sections
For disposal information, see Section 13. For protective clothing and equipment, see Section 8.
 
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
 
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area away from sources of heat or ignition. Protect from physical damage. Isolate from incompatible materials (section 10).
 
Incompatibles
Strong oxidizing agents, alkali, dichromates, alkali iodides, reducing agents.
 
7.3 Specific End Uses
Investigational research by professional users
 
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
ACGIH Threshold Limit Value (TLV): none established
OSHA Permissible Exposure Limit (PEL): none established
 
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
 
Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator.
 
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
 
Skin Protection
Wear protective gloves and clean body covering clothing.
 
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
a. AppearanceDark green powderb. OdorOdorless
c. Odor ThresholdN.A.d. pHBasic
e. Melting/Freezing Point (oC)105f. Boiling point (oC)Decomposes
g. Flash Point (oC)N.A.h. Evaporation RateN.A.
i. FlammabilityN.A.j. Upper/Lower Flammability or Explosive LimitsN.A.
k. Vapor PressureNegligiblel. Vapor Density (Air = 1)13
m. Relative Density1.230n. Water SolubilitySoluble
o. Partition Coefficient n-octanol/waterN.A.p. Autoignition Temperature (oC)N.A.
q. Decomposition Temperature (oC)N.A.r. ViscosityNo data available.
s. Explosive PropertiesN.A.t. Oxidizing PropertiesNot an oxidizer
 
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Not reactive under normal conditions of use.
 
10.2 Chemical Stability
Stable under ordinary conditions of use and storage.
 
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
 
10.4 Conditions to Avoid
Heat, flames, ignition sources, and incompatibles.
 
10.5 Incompatible Materials
Strong oxidizing agents, alkali, dichromates, alkali iodides, reducing agents.
 
10.6 Hazardous Decomposition Products
Hydrogen chloride, nitrogen oxides, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, sulfur oxides, including sulfur oxide and sufur dioxide.
 
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
1180. Investigated as a mutagen.
 
Dermal Rabbit LD50 (mg/kg)
0
 
Component Cancer List Status
 NTP Carcinogen 
 KnownAnticipatedIARC Category
METHYLENE BLUENoNoNone
 
Potential Health Effects
Inhalation
May cause methemoglobinemia, cyanosis, convulsions, tachycardia, dyspnea, and death. Can produce delayed pulmonary edema.
 
Ingestion
A burning sensation of the mouth may be noted following ingestion. May cause nauseau, vomiting, diarrhea, and gastritis.
 
Skin
Absorption into the body may cause cyanosis. May color the skin a bluish color. May cause photosensitization.
 
Eyes
May cause chemical conjuctivitis.
 
Carcinogenicity
Not listed as a known or possible carcinogen by ACGIH, NIOSH, OSHA, NTP or IARC.
 
Mutagenicity
DNA Adduct: Mouse, Ascites tumor = 70 umol/L.; DNA Adduct: Mammal - species unspecified Lymphocyte = 1830 nmol/L.
 
Reproductive Toxicity
Oral, rat: TDLo = 2500 mg/kg (femal 1-22 day (s) after conception) Fertility - post - implantation mortality
 
Teratogenic Effects
No information found.
 
Routes of Entry
Ingestion, inhalation, skin contact.
 
Target Organ Statement
Acute or chronic overexposure to this material or its components may cause adverse effects to the following: lungs, thorax, or blood.
 
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data
 
 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data
 
12.2 Persistence and Degradability
No data
 
12.3 Bioaccumulative Potential
No data
 
12.4 Mobility in Soil
No data
 
12.5 Results of PBT and vPvB Assessment
No data
 
12.6 Other Adverse Effects
None
 
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
 
SECTION 14 - TRANSPORT INFORMATION
  ADR/RID IATA IMO DOT
14.1 UN Number N.A.N.A.N.A.N.A.
14.2 Shipping NameNot regulated.Not regulated.Not regulated.Not regulated.
14.3 Hazard ClassN.A.N.A.N.A.N.A.
14.4 Packing GroupN.A.N.A.N.A.N.A.
14.5 Environmental HazardsN.A.N.A.N.A.N.A.
14.6 Special PrecautionsN.A.N.A.N.A.N.A.
 
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
 
SARA 311/312 Hazard Categories
ComponentFirePressureReactivityAcuteChronic
METHYLENE BLUENoNoNoYesNo
 
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
 
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.1
12/17/2013 - Released Version 1.0

NFPA Codes
Health 1   Flammability 0  Reactivity 0
 
 
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.