Version 3.0
Date revised: 4/26/2019
SAFETY DATA SHEETNational Diagnostics
Conforms to regulation (EC) no. EU 453/2010
 
SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING
1.1 Product Identifier
Product Name: TAE Buffer 50X Product Number: EC-872
 
1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against
Investigational research by professional users
 
1.3 Details of the Supplier of the Safety Data Sheet
Manufacturer
National Diagnostics
305 Patton Drive
Atlanta, GA 30036
(404) 699-2121
(800) 526-3867
info@nationaldiagnostics.com
 
1.4 Emergency Telephone Number
ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)
 
SECTION 2 - HAZARDS IDENTIFICATION
2.1 Classification of the Substance or Mixture
Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]
H315 - Skin Corrosion/Irritation (Category 2)
H320 - Serious Eye Damage/Eye Irritation (Category 2B)
H335 - Specific Target Organ Toxicity, Single Exposure (Category 3)
 
2.2 Label Elements
GHS LABEL ELEMENTS AND CLASSIFICATION
GHS Label Elements
Harmful 
WARNING
H315 - Causes skin irritation.
H320 - Causes eye irritation.
H335 - May cause respiratory irritation.
P260 - Do not breathe dust/fumes/gas/mist/vapors/spray.
P264 - Wash skin thoroughly after handling.
P302+P352 - IF ON SKIN: Wash with plenty of soap and water.
P308+P313 - IF exposed or concerned: Call a POISON CENTER or doctor/physician.
  
  
 
2.3 Other Hazards
None found.
 
SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS
3.2 Mixture
Chemical Names/Description
Aqueous solution of buffer salts (EDTA < 1%)
 
Component List
Component % Comp. CAS # EC # 1278/2008 Classification
Tris-Base24 77-86-1  201-064-4 H315, H319, H335
Acetic Acid5.7 64-19-7  200-580-7 H226, H314
Ethylenediaminetetraacetic acid, disodium salt dihydrate.74 6381-92-6  205-358-3 H332
 
SECTION 4 - FIRST AID MEASURES
4.1 Description of First Aid Measures
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.
 
Ingestion
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. Call a physician.
 
Skin
Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.
 
Eyes
Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
 
4.2 Most Important Symptoms and Effects, Both Acute and Delayed
Inhalation
Tris-Base:
Coughing, shortness of breath.
 
Acetic Acid:
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include coughing and shortness of breath.
 
Ingestion
Tris-Base:
Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse, blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.
 
Acetic Acid:
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
 
Skin
Tris-Base:
Redness, itching, and pain.
 
Acetic Acid:
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation with redness and pain.
 
Eyes
Tris-Base:
Redness, itching, and pain.
 
Acetic Acid:
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Symptoms may include irritation, redness, pain, and corneal damage.
 
4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed
Unknown/not applicable
 
SECTION 5 - FIRE FIGHTING MEASURES
5.1 Extinguishing media
Use media appropriate to the primary cause of fire.
 
5.2 Special Hazards Arising from the Substance/Mixture
Hazardous Combustion Products
Thermal decomposition products may include toxic oxides of nitrogen and carbon.
 
Hazardous Decomposition Products
Burning may produce carbon monoxide, carbon dioxide, nitrogen oxides.
 
Hazardous Polymerization
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
 
5.3 Advice for Firefighters
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.
 
5.4 Further Information
No data available.
 
SECTION 6 - ACCIDENTAL RELEASE MEASURES
6.1 Personal Precautions
Wear appropriate protective equipment as specified in Section 8.
 
6.2 Environmental Precautions
Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.
 
6.3 Methods and Materials for Containment and Cleaning Up
Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using absorbents. Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with copious amounts of water.
 
6.4 References to Other Sections
For disposal information, see Section 13. For protective clothing and equipment, see Section 8.
 
SECTION 7 - HANDLING AND STORAGE
7.1 Precautions for Safe Handling
Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
 
7.2 Conditions for Safe Storage (including any incompatibles)
Keep in a tightly closed container, stored in a cooled, dry, ventilated area.
 
Incompatibles
Tris-Base:
No incompatibility data found.
 
Acetic Acid:
Chromic acid, nitric acid, ethylene glycol, perchloric acid, phosphorous trichloride, oxidizers, sodium peroxide, strong caustics, most metals (except aluminum), carbonates, hydroxides, oxides, and phosphates.
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Oxidizing agents.
 
7.3 Specific End Uses
Investigational research by professional users
 
SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS
8.1 Control Parameters
Component: Tris-Base
ACGIH Threshold Limit Value (TLV): none established
OSHA Permissable Exposure Limit (PEL): none established
 
Component: Acetic Acid
ACGIH Threshold Limit Value (TLV): 10 ppm (TWA)
OSHA Permissable Exposure Limit (PEL): None established
 
Component: Ethylenediaminetetraacetic acid, disodium salt dihydrate
ACGIH Threshold Limit Value (TLV): None Established
OSHA Permissable Exposure Limit (PEL): None established
 
8.2 Exposure Controls
Engineering Controls
A system of local and/or general exhaust is recommended to keep employee exposures low. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.
 
Respiratory Protection
For conditions of use where exposure to the dust or mist is apparent, a full-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator.
 
Eye Protection
Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.
 
Skin Protection
Wear protective gloves and clean body covering clothing.
 
SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES
9.1 Information on Basic Physical & Chemical Properties
a. AppearanceClear, colorless solutionb. OdorNone
c. Odor ThresholdN.A.d. pH8.3
e. Melting/Freezing Point (oC)0f. Boiling point (oC)104.4
g. Flash Point (oC)N.A.h. Evaporation Rate1.0
i. FlammabilityN.A.j. Upper/Lower Flammability or Explosive LimitsN.A.
k. Vapor PressureWaterl. Vapor Density (Air = 1)N.A.
m. Relative Density.98n. Water SolubilitySoluble
o. Partition Coefficient n-octanol/waterMixturep. Autoignition Temperature (oC)N.A.
q. Decomposition Temperature (oC)N.A.r. ViscosityNo data available.
s. Explosive PropertiesN.A.t. Oxidizing PropertiesN.A.
 
SECTION 10 - STABILITY AND REACTIVITY
10.1 Reactivity
Not reactive under normal conditions of use and storage.
 
10.2 Chemical Stability
Stable under ordinary conditions of use and storage.
 
10.3 Possibility of Hazardous Reactions
Will not occur under normal conditions of use (See Sections 10.4 & 10.5).
 
10.4 Conditions to Avoid
Heat, incompatibles.
 
10.5 Incompatible Materials
Tris-Base:
No incompatibility data found.
 
Acetic Acid:
Chromic acid, nitric acid, ethylene glycol, perchloric acid, phosphorous trichloride, oxidizers, sodium peroxide, strong caustics, most metals (except aluminum), carbonates, hydroxides, oxides, and phosphates.
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate:
Oxidizing agents.
 
10.6 Hazardous Decomposition Products
Burning may produce carbon monoxide, carbon dioxide, nitrogen oxides.
 
SECTION 11 - TOXICOLOGICAL INFORMATION
Product LD50 Values
Oral Rat LD50 (mg/kg)
24583
 
Dermal Rabbit LD50 (mg/kg)
N.A.
 
Component Cancer List Status
 NTP Carcinogen 
 KnownAnticipatedIARC Category
Tris-BaseNoNoNone
Acetic AcidNoNoNone
Ethylenediaminetetraacetic acid, disodium salt dihydrateNoNoNone
 
Potential Health Effects
Inhalation
Tris-Base
Causes irritation to the respiratory tract.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes irritation of the mucous membrane and upper respiratory tract.
 
Ingestion
Tris-Base
Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Substance has low toxicity by ingestion. Large amounts may cause gastric upset due to osmotic imbalance through the sequestering of metal ions.
 
Skin
Tris-Base
Causes irritation to the skin.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes skin irritation. Causes redness and pain.
 
Eyes
Tris-Base
Causes irritation to the eyes.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Causes eye irritation. Causes redness and pain.
 
Carcinogenicity
Tris-Base
Not listed as a carcinogen by NTP or IARC.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Not listed by NTP or IARC as a known or anticipated carcinogen.
 
Mutagenicity
Tris-Base
No information found.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Cytogenetic Analysis: intraperitoneal - mouse 50mmol/L. DNA Inhibition: hamster fibroblast 500ug/L, rabbit kidney 250 umol/L.
 
Reproductive Toxicity
Tris-Base
No information found.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Fertility: Post-implantation mortality, oral - rat TDLo = 7632 mg/kg.
 
Teratogenic Effects
Tris-Base
No information found.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Embryo or Fetus: Stunted fetus, oral - rat TDLo = 7632 mg/kg. Specific developmental abnormalities: cardiovascular, craniofacial, musculoskeletal, respiratory, and urogenital, oral - rat TDLo = 7632 mg/kg.
 
Routes of Entry
Tris-Base
Ingestion.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Inhalation, ingestion or skin contact.
 
Target Organ Statement
Tris-Base
No information available.
 
Acetic Acid
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No information available
 
SECTION 12 - ECOLOGICAL INFOMATION
12.1 Toxicity
COMPONENT: Tris-Base
 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
LC50 460mg/l (Golden ide)EC50: 59.8 mg/L (Daphnia)EC50: 473mg/l @ 48 hrsCE50>1000mg/L (3hrs)

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data
 
COMPONENT: Acetic Acid
 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data
 
COMPONENT: Ethylenediaminetetraacetic acid, disodium salt dihydrate
 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
LC50 (96hr, Lepomis macrochirus ):41mg/LEC50 (485hr daphnia) 140mg/LEC 50 (72hrs)>100mg/LEC50>500mg/L

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataLOEC (Nicotiana tabacum ) 420mg/LNo data
 
12.2 Persistence and Degradability
Tris-Base
Readily Biodegradable (>97% degradation at 28 days)
 
Acetic Acid
No data
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Biodegradable (55% degradation in 20 days)
 
12.3 Bioaccumulative Potential
Tris-Base
No data
 
Acetic Acid
No data
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
BCF 1.1-1.8
 
12.4 Mobility in Soil
Tris-Base
Log Koc 1.57-1.85
 
Acetic Acid
No data
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
No data
 
12.5 Results of PBT and vPvB Assessment
Tris-Base
Not a PBT or vPvB
 
Acetic Acid
No data
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
Not PBT or vPvB
 
12.6 Other Adverse Effects
Tris-Base
None
 
Acetic Acid
None
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
None
 
SECTION 13 - DISPOSAL CONSIDERATIONS
13.1 Waste Treatment Methods
Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.
 
SECTION 14 - TRANSPORT INFORMATION
  ADR/RID IATA IMO DOT
14.1 UN Number N.A.N.A.N.A.N.A.
14.2 Shipping NameNot RegulatedNot RegulatedNot RegulatedNot Regulated
14.3 Hazard ClassN.A.N.A.N.A.N.A.
14.4 Packing GroupN.A.N.A.N.A.N.A.
14.5 Environmental HazardsN.A.N.A.N.A.N.A.
14.6 Special PrecautionsN.A.N.A.N.A.N.A.
 
SECTION 15 - REGULATORY INFORMATION
15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture
United States
TSCA Regulatory Statement
All intentional ingredients are listed on the TSCA Inventory.
 
SARA 311/312 Hazard Categories
ComponentFirePressureReactivityAcuteChronic
Tris-BaseNoNoNoYesNo
Acetic AcidYesNoYesYesYes
Ethylenediaminetetraacetic acid, disodium salt dihydrateNoNoNoYesNo
 
Europe
EEC Regulatory
All intentional ingredients are listed on the European EINECS Inventory.
 
SECTION 16 - OTHER INFORMATION
Revisional Updates
4/26/2019 - Updated Section 1.4
1/7/2016 - Updated Section 3.2
5/29/2015 - Updated Sections 2.1 and 3.2
8/21/2013 - Released Version 1.0

NFPA Codes
Health 1   Flammability 0  Reactivity 0
 
Dangers
Tris-Base
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.
 
Acetic Acid
H226 - Flammable liquid and vapor.
H314 - Causes severe skin burns and eye damage.
 
Ethylenediaminetetraacetic acid, disodium salt dihydrate
H332 - Harmful if inhaled.
 
 
MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.