Counting Carbon Dioxide by LSC

Prior to the introduction of Liquid Scintillation counting, a primary route of radiotracer analysis was to combust the organic material and detect the 14CO2 so generated in a gas phase proportional counter. Many protocols still call for combustion and 14CO2 counting, and many metabolic studies require quantitation of 14CO2 exhaled by tracer-fed animals. 14CO2 is assayed by trapping it in a liquid phase as a complex with a strong base (carbamate) and then counting the liquid phase.

Bubbling 14CO2 through carbamate traps the gas in the liquid phase, yielding a suitable sample for liquid scintillation counting.

Counting 14CO2

OXOSOL C14

This is a single solution containing both carbamate and scintillators. Gas containing 14CO2 is shaken with the cocktail or bubbled through with a sparger. It is advisable to extract the gas with a second volume of solution to ensure capture of >90% of the 14CO2. Cap the vials and count.

CARBAMATE-1 + OXOSOL 306

In this system, the carbamate is provided as a separate solution, which may enhance capture efficiencies. Shake or bubble the gas with 1ml of CARBAMATE-1. Add 10ml of OXOSOL 306 and count.

NEXT TOPIC: Preparing Samples in PAGE Gels for LSC

Products Related to this Discussion:

Oxosol C14
Complete scintillator designed to absorb and count 14CO2 produced by sample combustion.

Carbamate-1
High-capacity CO2 absorber intended to be used in conjunction with Oxosol 306.

Oxosol 306
Complete scintillation counting solution specifically formulated to count CO2 samples trapped in Carbamate.

SEARCH

NAVIGATION

APPLICATIONS

Top » National Diagnostics » LSC Principles & Methods » LSC Applications » Counting Carbon Dioxide by LSC

REQUEST A CATALOG

SHOPPING CART

CURRENCIES

NEW PRODUCTS